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Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain
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Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain

Live Imaging of Microtubule Dynamics in Glioblastoma Cells Invading the Zebrafish Brain

DOI:
10.3791/64093-v

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09:29 min

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July 29, 2022

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, ,
1Cell Polarity, Migration and Cancer Unit,Université Paris-Cité, Institut Pasteur, Équipe Labellisée Ligue Contre le Cancer

Chapters

  • 00:04Introduction
  • 01:03Xenotransplantation Procedure
  • 04:46Intravital Imaging of the Glioblastoma Xenografts
  • 07:41Results: Understanding Microtubule Dynamics of Glioblastoma Cells via Live Imaging of Zebrafish Larval Xenografts
  • 08:53Conclusion

Summary

Automatic Translation

Automatic Translation

We report a technique permitting live imaging of microtubule dynamics in glioblastoma (GBM) cells invading a vertebrate brain tissue. Coupling the orthotopic injection of fluorescently tagged GBM cells into a transparent zebrafish brain with high-resolution intravital imaging allows the measurement of cytoskeleton dynamics during in situ cancer invasion.

Tags

Keywords: Microtubule DynamicsGlioblastoma CellsZebrafish BrainSubcellular Intravital ImagingBrain Cancer InvasionProtein AnalysisCell TransplantationMicroinjectionAnesthetized Larvae

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