JoVE Journal
Neuroscience
Neuroscience
A subscription to JoVE is required to view this content.
使用MRI,组织清除和光片显微镜对完整的新生小鼠大脑进行全脑单细胞成像和分析
The JoVE video player is compatible with HTML5 and Adobe Flash. Older browsers that do not support HTML5 and the H.264 video codec will still use a Flash-based video player. We recommend downloading the newest version of Flash here, but we support all versions 10 and above.
If that doesn't help, please let us know.
Unable to load video. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
An unexpected error occurred. Please check your Internet connection and reload this page. If the problem continues, please let us know and we'll try to help.
Click here for the English version
Article
DOI:
10.3791/64096-v
•
08:49 min
•
August 1st, 2022
使用MRI,组织清除和光片显微镜对完整的新生小鼠大脑进行全脑单细胞成像和分析
August 1st, 2022
•
Felix A. Kyere*1,2, Ian Curtin*1,2, Oleh Krupa1,2, Carolyn M. McCormick1,2, Mustafa Dere3, Sarah Khan3,7, Minjeong Kim7, Tzu-Wen Winnie Wang4,5,6, Qiuhong He4,5,6, Guorong Wu3, Yen-Yu Ian Shih4,5,6, Jason L. Stein1,2
1UNC Neuroscience Center, University of North Carolina, Chapel Hill, 2Department of Genetics, University of North Carolina, Chapel Hill, 3Department of Psychiatry, University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging, The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center, The University of North Carolina at Chapel Hill, 6Department of Neurology, The University of North Carolina at Chapel Hill, 7Department of Computer Science, The University of North Carolina at Greensboro
* These authors contributed equally
Chapters
Summary
Please note that all translations are automatically generated.
Click here for the English version.
该协议描述了使用iDISCO +对完整小鼠大脑进行磁共振成像,清除和免疫标记的方法,然后详细说明了使用光片显微镜成像和使用NuMorph的下游分析。