In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus

DOI:

10.3791/64104-v

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07:03 min

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July 06, 2022

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Ryosuke Kamei, Shinji Urata, Hisato Maruoka, Shigeo Okabe

¹Department of Cellular Neurobiology, Graduate School of Medicine,The University of Tokyo

Chapters

00:04Introduction
00:36Implantation of the Observation Window
02:34Two‐Photon Microscopy Immediately After Surgery for Quality Check
03:55In Vivo Chronic Two‐Photon Imaging of Microglia Using CX3CR1‐GFP Mice
04:29Results: In Vivo Imaging of Microglia in CX3CR1‐GFP Mice
06:20Conclusion

Summary

Automatic Translation

This paper describes a method for chronic in vivo observation of the resting microglia in the mouse hippocampal CA1 using precisely controlled surgery and two-photon microscopy.

In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus

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