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Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
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Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Article DOI: 10.3791/64111-v • 08:26 min • August 23rd, 2022
August 23rd, 2022 •
1, 1, 1, 1, 1
1Department of Cellular Neurobiology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo

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Here, we describe a protocol combining adeno-associated virus injection with cranial window implantation for simultaneous imaging of microglial dynamics and neuronal activity in awake mice.

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Simultaneous Imaging Microglial Dynamics Neuronal Activity Awake Mice Protocol Surveillance Behavior Interaction With Neurons Motion Artifact Robust Head Fixation Restoration High Frame Rate AAV Injection Cranial Window Implantation Technically Demanding Failure Practice Injection Apparatus Glass Pipette 26-gauge Hamilton Syringe Liquid Paraffin Microinjector Analgesia Auxiliary Ear Bars Stereotaxic Instrument Dorsal Side Up Surgical Site Disinfecting The Surgical Area Incision Scalp Midline Primary Visual Cortex
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