Porcine Liver Transplantation Without Veno-Venous Bypass As an Extended Criteria Donor Model

Current issues in liver transplantation require the implementation of large animal models. This protocol describes the technique of porcine orthotopic liver transplantation without the use of a veno-venous bypass. This technique significantly reduces the logistical and technical complexity of the procedure.

Furthermore, by engrafting livers after 20 hours of static cold storage, our model simulates extended criteria donor conditions. Begin by placing the anesthetized donor pig in a supine position and fix the limbs at the base of the operation table with elastic bands. Scrub the skin with an antiseptic agent and cover the animal with sterile drapes.