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Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry
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Cancer Research
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JoVE Journal Cancer Research
Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry

Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry

DOI:
10.3791/64176-v

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14:01 min

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September 13, 2022

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, , ,
1Department of Molecular Genetics and Cell Biology,University of Chicago

Chapters

  • 00:04Introduction
  • 01:16Induction of Senescence by Chemotherapy Drugs in Cultured Cancer Cells
  • 03:29DDAO-Galactoside Staining of Senescence-Associated Beta-Galactosidase (SA-β-Gal) in Cell or Tumor Samples
  • 04:56Flow Cytometer Setup and Data Acquisition
  • 06:27Flow Cytometry Data Analysis
  • 09:01Results: Screening Tumors and Chemotherapy Agents for the Induction of Senescence
  • 13:06Conclusion

Summary

Automatic Translation

Automatic Translation

A protocol for fluorescent, flow cytometric quantification of senescent cancer cells induced by chemotherapy drugs in cell culture or in murine tumor models is presented. Optional procedures include co-immunostaining, sample fixation to facilitate large batch or time point analysis, and the enrichment of viable senescent cells by flow cytometric sorting.

Tags

Keywords: SenescenceSenescent CellsFlow CytometryFluorescent Probeβ-galactosidaseTumor CellsCell CultureCancer BiologyCell TreatmentCell HarvestingCell CountingCell Staining

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