Far-red Fluorescence
Far-red Fluorescence - Related Videos
03:14
Measuring Mast Cell Exocytosis via Neuropeptide Y Monomeric Red Fluorescent Protein Release
0 Views •
2025
This video demonstrates mast cell exocytosis induced by an activating reagent that includes a calcium ionophore, initiating the release of secretory granules containing the fluorescent reporter protein NPY-mRFP. Subsequently, we quantitatively assess the exocytosis by measuring the released NPY-mRFP using a fluorescence plate reader.
07:02
Fluorescence Micropipette Aspiration Assay to Investigate Red Blood Cell Mechanosensing
0 Views •
Cited by 1 •
2024
The exploration of cellular behavior under mechanical stress is pivotal for advances in cellular mechanics and mechanobiology. We introduce the Fluorescence Micropipette Aspiration (fMPA) technique, a novel method combining controlled mechanical stimulation with comprehensive analysis of intracellular signaling in single cells. This technique investigates new in-depth studies of live-cell mechanobiology.
01:23
Red Algae
0 Views •
2025
Red algae, also known as rhodophytes, are primarily found in marine environments, though some species inhabit freshwater and terrestrial ecosystems. These organisms exist in both unicellular and multicellular forms, with some multicellular varieties reaching macroscopic sizes.As phototrophic organisms, red algae contain chlorophyll a; however, their chloroplasts lack chlorophyll b. Instead, they possess phycobiliproteins, which serve as major light-harvesting pigments, similar to those found in...
10:27
Controlled Microfluidic Environment for Dynamic Investigation of Red Blood Cell Aggregation
0 Views •
Cited by 8 •
2015
The protocol described details an experimental procedure to quantify Red Blood Cell (RBC) aggregates under a controlled and constant shear rate, based on image processing techniques. The goal of this protocol is to relate RBC aggregate sizes to the corresponding shear rate in a controlled microfluidic environment.
14:01
Far-Red Fluorescent Senescence-Associated β-Galactosidase Probe for Identification and Enrichment of Senescent Tumor Cells by Flow Cytometry
0 Views •
Cited by 7 •
2022
A protocol for fluorescent, flow cytometric quantification of senescent cancer cells induced by chemotherapy drugs in cell culture or in murine tumor models is presented. Optional procedures include co-immunostaining, sample fixation to facilitate large batch or time point analysis, and the enrichment of viable senescent cells by flow cytometric sorting.
FAQs
Its longer-wavelength emission generally encounters less tissue autofluorescence and light scattering than visible dyes. This can increase the contrast between a labeled target and the surrounding biological material, making fluorescent signals easier to detect in cellular and tissue samples. In cancer research, that improved optical window supports clearer observation of tumor-associated structures and labeled cells.
The fluorescent molecule first absorbs higher-energy light delivered at an appropriate excitation wavelength, then releases lower-energy photons in the far-red range. Imaging must therefore provide suitable excitation while collecting the emitted light around 650–750 nm. Matching these optical conditions to the fluorophore helps researchers detect the intended signal rather than an unsuitable wavelength range.
Spectrally distinct fluorophores can produce distinguishable signals within the same experiment, allowing researchers to examine multiple labeled targets. This multiplexed analysis can connect different features of tumor biology or compare several indicators of treatment response. Far-red fluorescence contributes a separate optical channel that may complement other fluorophores used in the overall measurement.
Compared with visible dyes, far-red emission generally produces less interference from tissue autofluorescence and light scattering. The resulting signal can be easier to identify in cells and tissues, particularly when biological material contributes substantial background. This difference makes far-red labels useful when researchers need improved detection of tumor cells, biomarkers, or other labeled components.
A researcher selects a far-red fluorescent label for the target of interest, applies appropriate excitation light, and records the resulting emission in the far-red range. The label may identify tumor cells, attach to a biomarker through a fluorescent antibody, or follow a delivered drug. The measured signal then reveals the location or distribution of that labeled target.
This combination is useful when researchers want to visualize specific biomarkers in cells or tissues. The antibody provides target recognition, while the far-red label supplies the detectable optical signal after excitation. Because the emission generally experiences lower autofluorescence and scattering than visible signals, the approach can support clearer biomarker localization within complex cancer specimens.
Far-red labels can be used to track tumor cells or monitor the distribution of a delivered drug during imaging of living animals. Their longer-wavelength emission is advantageous in tissue because it generally reduces autofluorescence and scattering relative to visible dyes. Researchers can therefore follow labeled biological material in vivo and assess its localization or movement.