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Far-rød fluorescerende senescens-associeret β-galactosidase sonde til identifikation og berigelse af senescent tumorceller ved flow cytometri
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Far-rød fluorescerende senescens-associeret β-galactosidase sonde til identifikation og berigelse af senescent tumorceller ved flow cytometri

Article DOI: 10.3791/64176-v • 14:01 min • September 13th, 2022
September 13th, 2022 •
1, 1, 1, 1
1Department of Molecular Genetics and Cell Biology, University of Chicago

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En protokol for fluorescerende, flowcytometrisk kvantificering af senescente kræftceller induceret af kemoterapilægemidler i cellekultur eller i murine tumormodeller præsenteres. Valgfrie procedurer omfatter co-immunostaining, prøvefiksering for at lette analyse af store batch- eller tidspunkter og berigelse af levedygtige senescente celler ved flowcytometrisk sortering.

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Kræftforskning udgave 187 ældning kræft kemoterapi flowcytometri fluorescerende sonde multiplex
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