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Biochemistry

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Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting
 

Study of the Functions and Activities of Neuronal K-Cl Co-Transporter KCC2 Using Western Blotting

Article DOI: 10.3791/64179-v
December 9th, 2022

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Summary December 9th, 2022

The present protocol highlights the application of western blotting technique to study the functions and activities of neuronal K-Cl co-transporter KCC2. The protocol describes the investigation of KCC2 phosphorylation at kinase regulatory sites Thr906/1007 via western blotting. Also, additional methods to confirm KCC2 activity are briefly highlighted in this text.

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