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Assessment of Thermal Damage from Robot-Drilled Craniotomy for Cranial Window Surgery in Mice
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JoVE Journal Neuroscience
Assessment of Thermal Damage from Robot-Drilled Craniotomy for Cranial Window Surgery in Mice

Assessment of Thermal Damage from Robot-Drilled Craniotomy for Cranial Window Surgery in Mice

DOI:
10.3791/64188-v

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09:30 min

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November 11, 2022

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, , , ,
1Department of Biomedical Engineering,Case Western Reserve University, 2Advanced Platform Technology Center,Louis Stokes Cleveland VA Medical Center

Chapters

  • 00:05Introduction
  • 00:33Surgical Robot Hardware Setup and Software Preparation
  • 02:00Surgery and Skull Preparation
  • 03:17Evans Blue Tail Vein Injection
  • 03:57Surgical Robot Drilling Procedure
  • 06:33Thermocouple Evaluation
  • 07:31Results: Semi-Automated Bone Drilling of Cranial Windows to Mitigate Thermal Blood-Brain Barrier Damage
  • 09:00Conclusion

Summary

Automatic Translation

Automatic Translation

Cranial windows have become a ubiquitously implemented surgical technique to allow for intravital imaging in transgenic mice. This protocol describes the use of a surgical robot that performs semi-automated bone drilling of cranial windows and can help reduce surgeon-to-surgeon variability and partially mitigate thermal blood-brain barrier damage.

Tags

Thermal DamageRobot-drilled CraniotomyCranial Window SurgeryMiceVascular DamageSurgical RobotCranial InjectionsBregmaMotor CortexAntibioticsAnalgesicsAnesthesiaSterile TechniqueHydrogen PeroxideTail Vein Injection

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