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东方果蝇背侧芽孢杆菌CRISPR/Cas9诱变实验方案
JoVE Journal
Biology
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JoVE Journal Biology
Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis
DOI:

08:19 min

September 28, 2022

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Chapters

  • 00:05Introduction
  • 00:33Target Design and In Vitro Synthesis of sgRNA
  • 01:18Embryo Collection and Preparation
  • 02:39Microinjection of Embryos
  • 04:24Post-Injection Insect Rearing
  • 05:07Mutant Screening
  • 06:41Results: Mutant Screening in B. dorsalis
  • 07:39Conclusion

Summary

Automatic Translation

本文介绍了东方果蝇背CRISPR/Cas9诱变的分步方案。该标准化协议提供的详细步骤将作为产生突变果蝇的有用指南,用于背双歧杆菌的功能基因研究。

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