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Protocolos para la mutagénesis CRISPR/Cas9 de la mosca oriental de la fruta Bactrocera dorsalis
Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly <em>Bactrocera dorsalis</em>
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Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis

Protocolos para la mutagénesis CRISPR/Cas9 de la mosca oriental de la fruta Bactrocera dorsalis

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2,301 Views

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08:19 min

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September 28, 2022

DOI:

10.3791/64195-v

DOI:
10.3791/64195-v

•

08:19 min
September 28, 2022

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2263 Views
, , , , , ,
1Key Laboratory of Sustainable Forest Ecosystem Management – Ministry of Education,Northeast Forestry University2Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences

Summary

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Este artículo presenta los protocolos paso a paso para la mutagénesis CRISPR/Cas9 de la mosca oriental de la fruta Bactrocera dorsalis. Los pasos detallados proporcionados por este protocolo estandarizado servirán como una guía útil para generar moscas mutantes para estudios de genes funcionales en B. dorsalis.

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Cite this Article

Yuan, J., Zhang, J., Zhang, Y., QiQiGe, W., Liu, W., Yan, S., Wang, G. Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis. J. Vis. Exp. (187), e64195, doi:10.3791/64195 (2022).

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