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CRISPR / Cas9 Oryantal Meyve Sineği Bactrocera dorsalis'in mutagenezi için protokoller
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CRISPR / Cas9 Oryantal Meyve Sineği Bactrocera dorsalis'in mutagenezi için protokoller

Article DOI: 10.3791/64195-v • 08:19 min • September 28th, 2022
September 28th, 2022 •
1, 1, 1, 1, 2, 1, 2
1Key Laboratory of Sustainable Forest Ecosystem Management - Ministry of Education, Northeast Forestry University, 2Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences

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Bu yazıda, Oryantal meyve sineği Bactrocera dorsalis'in CRISPR / Cas9 mutagenezi için adım adım protokoller sunulmaktadır. Bu standartlaştırılmış protokol tarafından sağlanan ayrıntılı adımlar, B. dorsalis'teki fonksiyonel gen çalışmaları için mutant sineklerin üretilmesi için yararlı bir rehber görevi görecektir.

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