Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Microfluidics-Assisted Selective Depolarization…
JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content.

JoVE Journal Neuroscience
Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
Studying the Integration of Adult-born Neurons
Journal (Neuroscience)
Studying the Integration of Adult-born Neurons
A Protocol for Measuring Cue Reactivity in a Rat Model of Cocaine Use Disorder
Journal (Neuroscience)
A Protocol for Measuring Cue Reactivity in a Rat Model of Cocaine Use Disorder
 

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

Article DOI: 10.3791/64196-v • 06:55 min • August 4th, 2022
August 4th, 2022 •
*1,2, *1,2, 2,3,4
1TUM Medical Graduate Center, Technical University of Munich, 2Max Planck Institute of Neurobiology, 3TUM School of Medicine, Institute of Neuronal Cell Biology, Technical University of Munich, 4Munich Cluster for Systems Neurology
* These authors contributed equally

Chapters

Summary
Automatic Translation

The present protocol describes the seeding and staining of neuronal mitochondria in microfluidic chambers. The fluidic pressure gradient in these chambers allows for the selective treatment of mitochondria in axons to analyze their properties in response to pharmacological challenges without affecting the cell body compartment.

Tags

Microfluidics-assisted Selective Depolarization Axonal Mitochondria Neurodegenerative Diseases Fluidic Pressure Gradient Selective Treatment Cell Body Processes Axonal Biology Membrane Potential Sensitive Dye Neuronal Cell Types Six Well Plates Sterile Hood Sterile Double Distilled Water Microfluidic Chamber 80% Ethanol Glass Plate Dissociated Hippocampal Neurons
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter