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JoVE Journal Genetics
C. elegans Gonaddisseksjon og frysesprekk for immunfluorescens og DAPI-farging
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C. elegans Gonaddisseksjon og frysesprekk for immunfluorescens og DAPI-farging

Article DOI: 10.3791/64204-v • 06:04 min • September 16th, 2022
September 16th, 2022 •
*1, *1, 1, 1
1Department of Biological Sciences, University of Massachusetts Lowell
* These authors contributed equally

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Dette er en vanlig metode for C. elegans gonad disseksjon etterfulgt av fryse sprekk, som produserer germline prøver for immunfluorescens via antistofffarging, eller for enkel DAPI farging for å visualisere DNA. Denne protokollen har vært vellykket for studenter i et forskningslaboratorium og i en kursbasert grunnforskningserfaring.

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