Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / CRISPR-Cas9-Mediated Precise Knock-In Edits in…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts
Pulling Membrane Nanotubes from Giant Unilamellar Vesicles
Journal (Biology)
Pulling Membrane Nanotubes from Giant Unilamellar Vesicles
Organotypic Culture of Adult Rabbit Retina
Journal (Biology)
Organotypic Culture of Adult Rabbit Retina
 

CRISPR-Cas9-Mediated Precise Knock-In Edits in Zebrafish Hearts

Article DOI: 10.3791/64209-v • 06:52 min • September 13th, 2022
September 13th, 2022 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Department of Biomedical Physiology and Kinesiology, Simon Fraser University

Chapters

Summary
Automatic Translation

This protocol describes an approach to facilitate precise knock-in edits in zebrafish embryos using CRISPR-Cas9 technology. A phenotyping pipeline is presented to demonstrate the applicability of these techniques to model a Long QT Syndrome-associated gene variant.

Tags

CRISPR-Cas9 Knock-in Edits Zebrafish Hearts Precise Integration Visual Detection Post-edit Phenotyping Disease-associated Variants Long QT Syndrome Pharmaceutical Therapies SgRNA Guides Excise Knock-in Target Site Sequence Zebrafish Ortholog Ensembl Gene Sequence Of Interest Flanking Sequence Template Design Software Tool CRISPOR Danio Rerio Species Cas Enzyme SgRNA Approach Target Exon Downstream Intron Off-target Binding CRISPOR Rankings Potential Off-target Sites PCR-based Sanger Sequencing Genotyping
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter