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CRISPR-Cas9-medierte presise knock-in-redigeringer i sebrafiskhjerter
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CRISPR-Cas9-medierte presise knock-in-redigeringer i sebrafiskhjerter

Article DOI: 10.3791/64209-v • 06:52 min • September 13th, 2022
September 13th, 2022 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1
1Department of Biomedical Physiology and Kinesiology, Simon Fraser University

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Denne protokollen beskriver en tilnærming for å legge til rette for presise knock-in-redigeringer i sebrafiskembryoer ved hjelp av CRISPR-Cas9-teknologi. En fenotypingspipeline presenteres for å demonstrere anvendeligheten av disse teknikkene for å modellere en Long QT Syndrome-assosiert genvariant.

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Biologi utgave 187 CRISPR-Cas9 sebrafisk homologi-rettet reparasjon hERG Lang QT-syndrom
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