Journal
/
Immunology and Infection
/
Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

Determining Intestinal Permeability Using Lucifer Yellow in an Apical-Out Enteroid Model

DOI:
10.3791/64215-v

•

09:48 min

•

July 27, 2022

•

, , , , , ,
1Division of Pediatric Surgery,Oklahoma Children’s Hospital, 2Oklahoma University Health Sciences Center, 3Department of Surgery,University of Oklahoma Health Sciences Center

Chapters

  • 00:04Introduction
  • 00:59Generation of Apical‐Out (AO) Enteroids
  • 03:08Induction of Experimental Necrotizing Enterocolitis (NEC)
  • 05:06Measurement of Paracellular Permeability Utilizing Lucifer Yellow (LY)
  • 07:49Results: Determining the Intestinal Permeability Using Lucifer Yellow in an Apical‐Out Enteroid Model
  • 09:07Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol outlines a method that utilizes lucifer yellow in an apical-out enteroid model to determine intestinal permeability. This method can be used to determine paracellular permeability in enteroids that model inflammatory bowel diseases such as necrotizing enterocolitis.

Tags

Keywords: Lucifer YellowApical-out Enteroid ModelIntestinal PermeabilityParacellular PathwaysNecrotizing EnterocolitisCell Recovery SolutionCentrifugation3D Model24-well PlateAO Enteroids

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/64215?article_type=v

Determining the Reactivity and Titre of Serum using a Haemagglutination Assay
check_url/64215?article_type=v

Using Eggs from Schistosoma mansoni as an In vivo Model of Helminth-induced Lung Inflammation
check_url/64215?article_type=v

Enteric Bacterial Invasion Of Intestinal Epithelial Cells In Vitro Is Dramatically Enhanced Using a Vertical Diffusion Chamber Model
check_url/64215?article_type=v

Following in Real Time the Impact of Pneumococcal Virulence Factors in an Acute Mouse Pneumonia Model Using Bioluminescent Bacteria
check_url/64215?article_type=v

Establishment of a High-throughput Setup for Screening Small Molecules That Modulate c-di-GMP Signaling in Pseudomonas aeruginosa
check_url/64215?article_type=v

Analysis of Lymphocyte Extravasation Using an In Vitro Model of the Human Blood-brain Barrier
check_url/64215?article_type=v

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
check_url/64215?article_type=v

A Novel Human Epithelial Enteroid Model of Necrotizing Enterocolitis
check_url/64215?article_type=v

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
check_url/64215?article_type=v

Induced Differentiation of M Cell-like Cells in Human Stem Cell-derived Ileal Enteroid Monolayers

Read Article