/
/
Modeling Brain Metastasis by Internal Carotid Artery Injection of Cancer Cells
A subscription to JoVE is required to view this content. Sign in or start your free trial.
1UQ Centre for Clinical Research,The University of Queensland, 2Centre for Advanced Imaging, Australian Institute for Bioengineering and Nanotechnology and ARC Training Centre for Innovation in Biomedical Imaging Technology,The University of Queensland, 3School of Biomedical Sciences,The University of Queensland, 4Mater Research and Mater Research Institute,The University of Queensland, 5Pathology Queensland,Royal Brisbane Women’s Hospital
Chapters
- 00:04Introduction
- 01:06Preparation of Cancer Cells for Injection
- 03:01Preparation of the Mouse for the Procedure
- 04:06Internal Carotid Injection and Post-Injection Recovery
- 07:44Results: Generation of Brain Metastasis Murine Model for Screening and Evaluating the Therapeutic Profile of Drugs
- 09:23Conclusion
Brain metastasis is a cause of severe morbidity and mortality in cancer patients. Most brain metastasis mouse models are complicated by systemic metastases confounding analysis of mortality and therapeutic intervention outcomes. Presented here is a protocol for internal carotid injection of cancer cells that produces consistent intracranial tumors with minimal systemic tumors.
