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Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique
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JoVE Journal Biology
Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique

Estimating the Yield of Compounds on the TLC Plate via the Blue-LED Illumination Technique

DOI:
10.3791/64230-v

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06:14 min

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October 06, 2022

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,
1Department of Marine Biotechnology and Resources,National Sun Yat-sen University, 2Doctoral Degree Program in Marine Biotechnology,National Sun Yat-sen University

Chapters

  • 00:04Introduction
  • 00:46Compound Extraction and Separation of the Crude Extract by Normal Phase Adsorption Column
  • 03:04Preparation and Loading of Thin-Layer Chromatogram Plates and Analysis by the Blue-LED Illuminator
  • 04:28Results: Estimating the Yield of Compounds Using the Blue-LED Illumination Technique
  • 05:49Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol developed a method to estimate the yield of compounds on the TLC plate using the blue-LED illumination technique. The advantages of this approach are that it is safe, effective, inexpensive, and allows the researcher to measure multiple samples simultaneously.

Tags

Keywords: TLCBlue-LED IlluminationYield EstimationCompound ExtractionColumn ChromatographyLovastatinNatural Products Chemistry

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