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Journal / Genetics / 通过邻近连接和定量PCR检测酿酒酵母同源重组中间体…
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JoVE Journal Genetics
通过邻近连接和定量PCR检测酿酒酵母同源重组中间体
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通过邻近连接和定量PCR检测酿酒酵母同源重组中间体

Article DOI: 10.3791/64240-v • 07:55 min • September 11th, 2022
September 11th, 2022 •
1, 2, 2, 1,3
1Department of Microbiology & Molecular Genetics, University of California, Davis, 2Laboratory of Biology & Modeling of the Cell, École Normale Supérieure de Lyon, 3Department of Molecular & Cellular Biology, University of California, Davis

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D环捕获（DLC）和D环延伸（DLE）测定利用邻近连接原理与定量PCR一起量化酿 酒酵母中诱导双链断裂部位的D环形成，D环延伸和产物形成。

Tags

遗传学，第187期，D环，基因组稳定性，联合分子，Rad51，断裂诱导复制
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