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Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation
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Bioengineering
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JoVE Journal Bioengineering
Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

DOI:
10.3791/64259-v

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08:47 min

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September 28, 2022

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, , , ,
1Department of Microbiology and Immunology,The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, 2Department of Infectious Diseases,The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, 3Victorian Infectious Diseases Service,Royal Melbourne Hospital at the Peter Doherty Institute for Infection and Immunity, 4Department of Infectious Diseases,Alfred Hospital and Monash University, 5Department of Chemical Engineering,The University of Melbourne, 6Department of Biomedical Engineering,The University of Melbourne

Chapters

  • 00:05Introduction
  • 00:45Preparation
  • 01:58Measurement
  • 03:53Analysis
  • 05:11Determination of the Absolute Fluorescence Intensity per Carrier
  • 05:56Cell Experiment
  • 07:08Results: A Two-Stream Methodology to Convert Relative, Semiquantitative Flow Cytometry Cell-Carrier Association Data into Absolute Quantification
  • 08:17Conclusion

Summary

Automatic Translation

Automatic Translation

A workflow is demonstrated for the absolute quantification of drug carrier-cell interactions using flow cytometry to allow better rational evaluation of novel drug delivery systems. This workflow is applicable to drug carriers of any type.

Tags

Keywords: Quantitative AnalysisDrug Delivery SystemsCellsIn VitroNanoparticle Tracking AnalysisCarrier SystemsPreclinical Nanomedicine EvaluationCarrier PerformanceLinear RangeQuantitative RangeFlow CellCarrier DebrisCarrier SuspensionCarrier DilutionCarrier Concentration

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