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JoVE Journal Bioengineering
Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro
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Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

Article DOI: 10.3791/64259-v • 08:47 min • September 28th, 2022
September 28th, 2022 •
1, 2, 2,3,4, 5, 6
1Department of Microbiology and Immunology, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, 2Department of Infectious Diseases, The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, 3Victorian Infectious Diseases Service, Royal Melbourne Hospital at the Peter Doherty Institute for Infection and Immunity, 4Department of Infectious Diseases, Alfred Hospital and Monash University, 5Department of Chemical Engineering, The University of Melbourne, 6Department of Biomedical Engineering, The University of Melbourne

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Summary
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A workflow is demonstrated for the absolute quantification of drug carrier-cell interactions using flow cytometry to allow better rational evaluation of novel drug delivery systems. This workflow is applicable to drug carriers of any type.

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Experimental Quantification Interactions Drug Delivery Systems Cells In Vitro Preclinical Nanomedicine Evaluation Protocol Quantitative Data Engineering Optimization Carrier Systems Qualitative Results Quantitative Results Carrier Dilution Linear Range Nano Side Flow Cell Laser Module Distilled Water Bubbles Air-liquid Interface Camera Capture Settings Tab Static Carriers
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