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Journal / Developmental Biology / Fluorescerende in situ hybridisering og…
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Developmental Biology

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JoVE Journal Developmental Biology
Fluorescerende in situ hybridisering og 5-ethynyl-2'-deoxyuridin mærkning for stamlignende celler i hydrozoan vandmænd Cladonema pacificum
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Fluorescerende in situ hybridisering og 5-ethynyl-2'-deoxyuridin mærkning for stamlignende celler i hydrozoan vandmænd Cladonema pacificum

Article DOI: 10.3791/64285-v • 08:44 min • August 3rd, 2022
August 3rd, 2022 •
1, 1, 2, 2
1Graduate School of Life Sciences, Tohoku University, 2Graduate School of Pharmaceutical Sciences, The University of Tokyo

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Her beskriver vi en protokol til visualisering af stammelignende prolifererende celler i vandmændene Cladonema. Helmonteret fluorescerende in situ-hybridisering med en stamcellemarkør muliggør påvisning af stamlignende celler, og 5-ethynyl-2'-deoxyuridinmærkning muliggør identifikation af prolifererende celler. Sammen kan aktivt prolifererende stamlignende celler detekteres.

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Udviklingsbiologi udgave 186 FISK EdU vandmænd medusa Cladonema pacificum stamceller celleproliferation
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