Stem Cell Marker
Stem Cell Marker - Related Videos
22:06
Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells
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Cited by 8 •
2007
This protocol details the derivation of transplantable hematopoietic stem cells from mouse embryonic stem cells (ESC) and their subsequent injection into lethally irradiated recipient mice. Briefly, ESC are differentiated as embryoid bodies, which are then infected with retroviral HoxB4 and co-cultured with OP9 stromal cells and hematopoietic cytokines.
10:32
Enumeration of Neural Stem Cells Using Clonal Assays
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Cited by 5 •
2016
Neural stem cells (NSCs) refer to cells which can self-renew and differentiate into the three neural lineages. Here, we describe a protocol to determine NSC frequency in a given cell population using neurosphere formation and differentiation under clonal conditions.
11:39
Identifying Cell Surface Markers of Primary Neural Stem and Progenitor Cells by Metabolic Labeling of Sialoglycan
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Cited by 1 •
2019
Presented here is a protocol that combines an in vitro neural-endothelial co-culture system and metabolic incorporation of sialoglycan with bioorthogonal functional groups to expand primary neural stem and progenitor cells and label their surface sialoglycoproteins for imaging or mass-spectrometry analysis of cell surface markers.
08:25
Reprogramming Human Somatic Cells into Induced Pluripotent Stem Cells (iPSCs) Using Retroviral Vector with GFP
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Cited by 6 •
2012
A method to generate human induced pluripotent stem cells (iPSCs) via retrovirus-mediated ectopic expression of OCT4, SOX2, KLF4 and MYC is described. A practical way to identify human iPSC colonies based on GFP expression is also discussed.
09:02
Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)
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Cited by 45 •
2013
This protocol describes the development, expansion, and in vivo imaging of NK cells derived from hESCs and iPSCs.
FAQs
A single marker rarely establishes stem-like identity because marker expression can reflect identity, potency, or activation without proving functional stemness. Researchers therefore examine combinations that provide a more informative profile and help distinguish stem-like cells from differentiated populations. This approach supports more careful characterization and reduces the risk of interpreting one molecular signal as conclusive evidence.
Cell-surface proteins can help distinguish and isolate cell populations because antibodies can detect them on intact cells. Transcription factors provide information about gene-regulatory states associated with cellular identity or potency. Measuring both types can give complementary evidence, while the choice of analysis depends on whether the goal is population separation, cellular characterization, or assessment of gene expression.
Marker profiles vary with species, tissue, and culture conditions, so the same marker pattern may not have identical significance in every experiment. Changes in the cellular environment can influence identity or activation state and alter measured expression. Researchers must therefore interpret profiles within the specific biological system rather than apply one universal marker set across all samples.
Researchers select markers relevant to the cell type and experimental question, then measure them with antibodies, flow cytometry, immunostaining, or gene-expression analysis. These methods provide different forms of evidence, such as population-level detection, visual localization, or molecular expression data. Combining compatible measurements helps characterize cells more rigorously than relying on a single assay.
Stem cell markers support the isolation and characterization of embryonic, adult, and induced pluripotent stem cells. They can help distinguish desired populations from differentiated cells and assess whether a culture retains an expected cellular profile. The resulting information is useful when studying differentiation or tissue development and when determining how cell populations change during an experiment.
In cell-based therapy studies, marker profiles help evaluate the cells being investigated, while organoid research uses them to characterize modeled cell states and developmental features. Disease studies can examine altered cellular identities or differentiation patterns through marker measurements. Because markers do not independently prove stemness, these applications require careful interpretation alongside the biological context and experimental conditions.