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Preparation of a Non-Cardiomyocyte Cell Suspension for Single-Cell RNA Sequencing from a Post-Myocardial Infarction Adult Mouse Heart

Article DOI: 10.3791/64290-v • 05:54 min • February 3rd, 2023
February 3rd, 2023 •
1,2, 1,2, 1,2, *1,2, *1,2
1Key Laboratory of Cardiovascular Intervention and Regenerative Medicine of Zhejiang Province, 2Department of Cardiology, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University Hangzhou
* These authors contributed equally

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Summary
Automatic Translation

Here, we describe a protocol to isolate a sufficient amount of single non-cardiomyocytes with high viability from a post-myocardial infarction (MI) mouse heart. This can be used for subsequent single-cell sequencing, flow cytometry analysis, and primary cell culture.

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Non-cardiomyocyte Cell Suspension Single-cell RNA Sequencing Myocardial Infarction Adult Mouse Heart Cell Viability Isolation Efficiency Low Costs Laboratory Equipment Heterogeneity Cardiac Pathophysiology Basic Studies Clinical Studies Heart Disease Post-MI Cardiovascular Environment Immune Cells Sample Preparation Pepsin Digestion Time Euthanized Mouse Supine Position Surgical Table Abdominal Skin Muscles Liver Avoidance Thorax Opening Left Ventricle Perfusion Fluid Excess Tissue Removal
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