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In vitro Cellulær aktivitetsevaluering af nanoemulsionsvaccineadjuvansen ophiopogonin D
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Immunology and Infection
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JoVE Journal Immunology and Infection
In Vitro Cellular Activity Evaluation of the Nanoemulsion Vaccine Adjuvant Ophiopogonin D

In vitro Cellulær aktivitetsevaluering af nanoemulsionsvaccineadjuvansen ophiopogonin D

DOI:
10.3791/64291-v

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08:15 min

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December 09, 2022

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, , , , , , , , , , , ,
1Department of Microbiology and Biochemical Pharmacy, National Engineering Research Centre of Immunological Products, College of Pharmacy,Third Military Medical University of Chinese PLA, 2Institute of Combined Injury of PLA, College of Military Preventive Medicine,Third Military Medical University of Chinese PLA

Chapters

  • 00:05Introduction
  • 00:55L929 Cytotoxicity Assay
  • 01:51Splenocyte Stimulation
  • 04:02Uptake by DCs
  • 06:09Macrophage Activation
  • 06:57Results: Impact of Adjuvant-Formulated Antigen on the Activation of PMs
  • 07:52Conclusion

Summary

Automatic Translation

Automatic Translation

Protokollen præsenterer detaljerede metoder til evaluering af, om nanoemulsionen ophiopogonin D-adjuvans fremmer effektive cellulære immunresponser.

Tags

In VitroCellular ActivityNanoemulsionVaccine AdjuvantOphiopogonin DCellular ResponseAdjuvant EvaluationL929 CellsDMEMCentrifugationMouse SpleenRed Blood Cell LysisCellular Delivery

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