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A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer
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Cancer Research
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JoVE Journal Cancer Research
A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

A Flow Cytometry-Based Cell Surface Protein Binding Assay for Assessing Selectivity and Specificity of an Anticancer Aptamer

DOI:
10.3791/64304-v

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10:46 min

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September 13, 2022

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, , , , ,
1School of Medicine,Deakin University, 2Institute for Mental and Physical Health and Clinical Translation, School of Medicine,Deakin University

Chapters

  • 00:05Introduction
  • 01:02Binding Assay
  • 04:10Flow Cytometry and Data Analysis
  • 08:58Results: Flow Cytometric-Based Assay to Study the Binding of an Aptamer and Cancer Cells
  • 09:55Conclusion

Summary

Automatic Translation

Automatic Translation

A necessary step in anticancer aptamer development is to test its binding to the target. We demonstrate a flow cytometric-based assay to study this binding, emphasizing the importance of including a negative control aptamer and cancer cells that are positive or negative for that particular protein. 

Tags

Keywords: Flow CytometryCell Surface ProteinAptamerAnticancerSelectivitySpecificityImmunophenotypingMulti-parametricCell CultureTrypsinizationCell CountingCell StabilizationBlocking BufferAptamer Folding

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