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JoVE Journal Neuroscience
Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity
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Brain Ventricular Microinjections of Lipopolysaccharide into Larval Zebrafish to Assess Neuroinflammation and Neurotoxicity

Article DOI: 10.3791/64313-v • 07:31 min • August 23rd, 2022
August 23rd, 2022 •
1, 1,2
1State Key Laboratory of Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences, University of Macau, Avenida da Universidade, 2Department of Pharmaceutical Sciences, Faculty of Health Sciences, University of Macau, Avenida da Universidade

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Summary
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This protocol demonstrates the microinjection of lipopolysaccharide into the brain ventricular region in a zebrafish larval model to study the resulting neuroinflammatory response and neurotoxicity.

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Brain Ventricular Microinjections Lipopolysaccharide Larval Zebrafish Neuroinflammation Neurotoxicity In Vivo Imaging Anti-neuroinflammatory Drugs Glass Capillaries Micropipette Puller Mineral Oil Microinjection Apparatus Micromanipulator Paraffin Oil Injection Solution PBS
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