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纳米巴支持的脂质双层系统,用于体外研究膜曲率传感蛋白
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro
DOI:

08:27 min

November 30, 2022

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Chapters

  • 00:04Introduction
  • 00:37Cleaning of Nanochip
  • 01:54Generation of Small Unilamellar Vesicles (SUVs)
  • 02:35Formation of the SLB on a Nanochip
  • 03:53Imaging the SLB and the Curvature Sensing Protein Binding on the Nanochip
  • 05:52Results: Protein Curvature Sensing Characterization and Membrane‐Binding Dynamics of Proteins Around the Nanobar
  • 07:43Conclusion

Summary

Automatic Translation

在这里,开发了一种纳米巴支持的脂质双层系统,以提供具有定义曲率的合成膜,从而能够在 体外表征具有曲率传感能力的蛋白质。

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