JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biochemistry
/
纳米巴支持的脂质双层系统,用于
体外研究
膜曲率传感蛋白
JoVE Journal
Biochemistry
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biochemistry
A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins
in vitro
Please note that all translations are automatically generated.
Click here for the English version.
纳米巴支持的脂质双层系统,用于
体外研究
膜曲率传感蛋白
DOI:
10.3791/64340-v
•
08:27 min
•
November 30, 2022
•
Xinwen Miao*
1
,
Jiawei Wu*
1,2
,
Wenting Zhao
3
1
School of Chemistry, Chemical Engineering, and Biotechnology
,
Nanyang Technological University
,
2
State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering
,
Zhejiang University of Technology
,
3
Institute for Digital Molecular Analytics and Science
,
Nanyang Technological University
Chapters
00:04
Introduction
00:37
Cleaning of Nanochip
01:54
Generation of Small Unilamellar Vesicles (SUVs)
02:35
Formation of the SLB on a Nanochip
03:53
Imaging the SLB and the Curvature Sensing Protein Binding on the Nanochip
05:52
Results: Protein Curvature Sensing Characterization and Membrane‐Binding Dynamics of Proteins Around the Nanobar
07:43
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这里,开发了一种纳米巴支持的脂质双层系统,以提供具有定义曲率的合成膜,从而能够在
体外表征
具有曲率传感能力的蛋白质。
Tags
Nanobar-supported Lipid Bilayer
Membrane Curvature Sensing
Protein Dynamics
Nanofabrication
Piranha Solution
Lipid Mixture
SUVs
PDMS Chamber
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
使用脚手架脂质体重构脂质近端蛋白质 - 蛋白质相互作用<em>体外</em
氮气气蚀和差速离心允许监测外周血膜蛋白在细胞中的分布
无洗涤剂超快重组膜蛋白成脂双层使用融合补充充电 Proteoliposomes。
用石英谐振器研究磷脂结合蛋白附件 a2 与固体支持的脂质层结合动力学的耗散微重法
细胞细胞接触物蛋白质-蛋白质相互作用的荧光波动光谱测定
电化学和荧光显微镜在质子泵膜酶研究中的单脂质体测定
利用体外荧光共振能量转移研究毫秒时间尺度蛋白质配合物的动力学
从鸡蛋黄中分离脂蛋白颗粒,研究细菌病原体脂肪酸并入膜磷脂
PeptiQuick,将膜蛋白一步进生物仿入肽,用于简化蛋白质结合测定
用于膜蛋白-蛋白质相互作用分析的原生细胞膜纳米粒子系统
Read Article