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JoVE Journal
Immunology and Infection
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Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major
 

Deciphering the Molecular Mechanism and Function of Pore-Forming Toxins Using Leishmania major

Article DOI: 10.3791/64341-v 08:17 min October 28th, 2022
October 28th, 2022

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Summary

Presented here is a protocol using Leishmania major promastigotes to determine the binding, cytotoxicity, and signaling induced by pore-forming toxins. A proof-of-concept with streptolysin O is provided. Other toxins can also be used to leverage the genetic mutants available in L. major to define new mechanisms of toxin resistance.

Tags

Keywords: Leishmania Major Pore-forming Toxins Cytotoxicity Assay Membrane-perturbing Agents Amphotericin B Propidium Iodide Flow Cytometry Serial Dilution Promastigotes Membrane Damage Cell Viability
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