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October 06, 2022
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The present protocol demonstrates the use of a fast, 3-stage, aptamer-based exponential amplification assay to detect targets. Sample preparation, signal amplification, and color development are covered to implement this system to recognize the presence of theophylline over that of caffeine.
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Cite this Article
Liao, A. M., Thoa, T. T. T., Caltagirone, G. T. Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction. J. Vis. Exp. (188), e64342, doi:10.3791/64342 (2022).
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