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Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction
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Bioengineering
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JoVE Journal Bioengineering
Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

Aptamer-Based Target Detection Facilitated by a 3-Stage G-Quadruplex Isothermal Exponential Amplification Reaction

DOI:
10.3791/64342-v

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03:38 min

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October 06, 2022

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, ,
1Aptagen LLC

Chapters

  • 00:04Introduction
  • 00:44Preparation of Ribozyme
  • 01:44GQ-EXPAR
  • 02:18Color Development
  • 02:45Results: GQ-EXPAR Visual Results
  • 03:12Conclusion

Summary

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The present protocol demonstrates the use of a fast, 3-stage, aptamer-based exponential amplification assay to detect targets. Sample preparation, signal amplification, and color development are covered to implement this system to recognize the presence of theophylline over that of caffeine.

Tags

Keywords: AptamerTarget DetectionG-quadruplexIsothermal Exponential AmplificationRibozymeT4 Polynucleotide KinaseTheophyllineCaffeineRNANicase PolymeraseEXPARHeminTMBAbsorbanceLow ResourceQuantification

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