Tracking Bispecific Antibody-Induced T Cell Trafficking Using Luciferase-Transduced Human T Cells

This method allows users to easily track T cells in vivo to evaluate the kinetics of homing and to monitor T cell persistence. The main advantage of this technique is that it can be repeated at multiple time points on the same animal without requiring sacrifice in flow cytometry or immunohistochemistry. This technique could be modified for tracking other types of immune cells as well.

To culture 293T cells, prepare one liter of DMEM by adding 110 milliliters of heat-inactivated fetal bovine serum and 11 milliliters of penicillin streptomycin. Thaw five times 10 to the sixth 293T cells and transfer them into a T175 flask containing DMEM. Split the cells one to 10 every three days for six days before the cells become more than 90%confluent.