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JoVE Journal
Bioengineering

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Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization
 

Cellular Affinity of Particle-Stabilized Emulsion to Boost Antigen Internalization

Article DOI: 10.3791/64406-v 10:06 min September 2nd, 2022
September 2nd, 2022

Chapters

Summary

To rationally design efficient adjuvants, we developed poly-lactic-co-glycolic acid nanoparticle-stabilized Pickering emulsion (PNPE). The PNPE possessed unique softness and a hydrophobic interface for potent cellular contact and offered high-content antigen loading, improving the cellular affinity of the delivery system to antigen-presenting cells and inducing efficient internalization of antigens.

Tags

PLGA Nanoparticles Pickering Emulsion Cellular Affinity Antigen Internalization Efficient Delivery Formulation Design Efficient Vaccines Polylactic-co-glycolic Acid Oil Phase PVA Aqueous Solution Syringe Pump Centrifuge Washing Process PLGA Nanoparticle Characterization Size Characterization Zeta Potential Characterization
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