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Developmental Biology
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小鼠卵母细胞质微管组织中心的多光子激光消融
JoVE Journal
Developmental Biology
This content is Free Access.
JoVE Journal
Developmental Biology
Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
Please note that all translations are automatically generated.
Click here for the English version.
小鼠卵母细胞质微管组织中心的多光子激光消融
DOI:
10.3791/64439-v
•
08:24 min
•
November 11, 2022
•
Daniela Londoño-Vásquez
2
,
Alexander Jurkevich
,
Ahmed Z. Balboula
1
Animal Sciences Research Center
,
University of Missouri
,
2
Universidad Nacional de Colombia
,
3
Advanced Light Microscopy Core
,
University of Missouri
Chapters
00:05
Introduction
01:12
Mouse Oocyte Collection
02:53
Oocyte Microinjection
04:16
Oocyte Maturation
05:02
Microscope Preparation for Ablation
06:05
Ablation of Metaphase Cytoplasmic Microtubule Organizing Centers (mcMTOCs)
06:59
Results: Depletion of Metaphase Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
07:35
Conclusion
Summary
Automatic Translation
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提出了一种优化的方案,该方案能够使用近红外飞秒激光在中期I期间消耗小鼠卵母细胞中的细胞质微管组织中心。
Tags
Multi-photon Laser Ablation
Cytoplasmic Microtubule Organizing Centers
Mouse Oocytes
Spindle Positioning
Meiosis
Aneuploidy
MTOCs
Polar MTOCs
McMTOCs
Targeted Ablation
Live Cell
Phototoxicity
Mechanical Perturbation
Cell Viability
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