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Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice
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Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

Differentiation and Imaging of Brown Adipocytes from the Stromal Vascular Fraction of Interscapular Adipose Tissue from Newborn Mice

DOI:
10.3791/64604-v

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04:46 min

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February 03, 2023

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, , ,
1Department of Nutrition, Diabetes and Metabolism,Pontificia Universidad Católica de Chile

Chapters

  • 00:00Introduction
  • 00:59Tissue Extraction
  • 01:36Tissue Digestion and Processing
  • 02:43Results: A Two-Phase Differentiation Procedure
  • 04:15Conclusion

Summary

Automatic Translation

Automatic Translation

Preadipocytes are isolated from the stromal vascular fraction of interscapular brown adipose tissue from newborn mice and differentiated into cells that accumulate lipid droplets, express molecular markers, and show the mitochondrial morphology of mature brown adipocytes. These cells are further analyzed by immunofluorescence and transmission electron microscopy.

Tags

BiologyStromal Vascular FractionNewborn MiceUncoupling Protein 1UCP1Mitochondrial ContentCell DifferentiationConfocal ImmunofluorescenceTransmission Electron Microscopy

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