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Application of Flow Vermimetry for Quantification and Analysis of the Caenorhabditis elegans Gut Microbiome
JoVE Journal
Biology
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JoVE Journal Biology
Application of Flow Vermimetry for Quantification and Analysis of the Caenorhabditis elegans Gut Microbiome
DOI:

08:38 min

March 31, 2023

Chapters

  • 00:05Introduction
  • 00:39Collecting Worm Population for Gut Microbiome Analyses
  • 02:08Setting Up the Large Particle Sorter and Autosampler
  • 03:43Sorting of Caenorhabditis elegans by Gut Microbiome Features
  • 06:05Results: Profiling Gut Microbiome Colonization
  • 07:50Conclusion

Summary

Automatic Translation

Caenorhabditis elegans is a powerful model to examine the molecular determinants driving host-microbiome interactions. We present a high throughput pipeline profiling the single animal levels of gut microbiome colonization together with key aspects of the C. elegans physiology.

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