Testing Cancer Immunotherapeutics in a Humanized Mouse Model Bearing Human Tumors

This protocol outlines the generation of Human Immune System, or HIS mice for preclinical immuno-oncology studies. Mouse cancer models are limited in their diversity and translate poorly to the clinic. This technique serves as a preclinical model for immunotherapy studies by evaluating the treatment of human tumors in a mouse with a human immune system.

The humanized mouse model can be used to study multiple aspects of human immunology, including responses to many infections, or cancers of the human immune system. Acquiring a reliable and robust source of cord blood and maintaining the health of the immunodeficient mouse colony can be challenging. Expertise in flow cytometry and immunological analysis is essential.