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Journal / Cancer Research / 从正常和肿瘤乳腺组织中生成和成像小鼠和人上皮类器官，无需传代…
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JoVE Journal Cancer Research
从正常和肿瘤乳腺组织中生成和成像小鼠和人上皮类器官，无需传代
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从正常和肿瘤乳腺组织中生成和成像小鼠和人上皮类器官，无需传代

Article DOI: 10.3791/64626-v • 08:57 min • November 11th, 2022
November 11th, 2022 •
1,2, 2,3,4,5, 1,2, 1,2, 1,2, 6, 1,2, 2,3,4,5, 1,2,3,4
1Department of Internal Medicine, Division of Hematology and Oncology, University of Texas Southwestern Medical Center, 2Harold C. Simmons Comprehensive Cancer Center, University of Texas Southwestern Medical Center, 3Department of Molecular Biology, University of Texas Southwestern Medical Center, 4Hamon Center for Regenerative Science and Medicine, University of Texas Southwestern Medical Center, 5Department of Cell Biology, University of Texas Southwestern Medical Center, 6Division of Medical Oncology, Norris Comprehensive Cancer Center, Keck School of Medicine, University of Southern California

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该协议讨论了通过差异离心从原发性正常和肿瘤乳腺组织产生上皮类器官的方法。此外，还包括用于三维培养以及嵌入类器官的免疫荧光成像的说明。

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癌症研究，第189期，
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