In Mycoplasma pneumoniae infection, serology tests can generate good results, yet with low specificity because of immunological cross-reaction. The in-house antigen-capture ELISA, described in this paper, guarantees high species specificity and has been shown to be a reliable screening test for accurate diagnosis of M. pneumoniae.
Yacoub, E., Chniba, I., Khadraoui, N., Mlik, B., Ben Abdelmoumen Mardassi, B. Antigen-Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Mycoplasma pneumoniae. J. Vis. Exp. (192), e64645, doi:10.3791/64645 (2023).