Antigen Capture Elisa
Antigen Capture Elisa - Related Videos
10:06
The ELISA Method
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2023
An enzyme-linked immunosorbent assay (ELISA) is typically performed to detect the presence and/or amount of a target protein of interest within an experimental sample. Detection of the target protein is made possible by antibodies, which make the ELISA an immunoassay. Through a series of incubation and washing steps, these antibodies, which are frequently linked, or conjugated, to an enzyme, will detect protein coating the bottom of a well on a microtiter plate. When exposed to a substrate,...
14:22
ELISA Assays: Indirect, Sandwich, and Competitive
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2023
Source: Whitney Swanson1,2, Frances V. Sjaastad2,3, and Thomas S. Griffith1,2,3,4 1 Department of Urology, University of Minnesota, Minneapolis, MN 55455 2 Center for Immunology, University of Minnesota, Minneapolis, MN 55455 3 Microbiology, Immunology, and Cancer Biology Graduate Program, University of Minnesota, Minneapolis, MN 55455 4 Masonic Cancer Center, University of Minnesota, Minneapolis, MN 55455 Enzyme-linked immunosorbent assay (ELISA) is frequently used to measure the presence...
14:48
Conversion of a Capture ELISA to a Luminex xMAP Assay using a Multiplex Antibody Screening Method
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Cited by 53 •
2012
An ELISA can be easily converted to a Luminex xMAP assay and, through the benefits of multiplexing, several antibodies can be screened simultaneously to identify an optimum antibody pair, resulting in increased sensitivity and dynamic range, while reducing assay cost.
03:55
ELISA-Based Method to Analyze Interactions between Bait and Prey Proteins
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2025
This video describes an ELISA-based technique that detects protein-protein interactions between two proteins by measuring the binding of the second protein to the immobilized first protein. The bound second protein is detected using specific antibodies labeled with an enzyme, such as horseradish peroxidase.
02:57
Enzyme-Linked Immunosorbent Assay to Verify Chemically-Coupled Antibody-Antigen Conjugates
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2025
This video demonstrates the detection of chemically conjugated antibody-antigen complexes via the sandwich enzyme-linked immunosorbent assay, or ELISA, technique. The captured antibody binds to the specific epitope of the antigen of the antibody-antigen conjugate, which can be later visualized using an enzyme-linked secondary antibody.
FAQs
The two-antibody arrangement improves analytical selectivity because the target must be recognized through both a capture antibody and a detection antibody. The first antibody retains the antigen on the microplate, while the second identifies the captured material and carries the enzyme-linked label. This paired-recognition design helps distinguish the target from other proteins in a biological sample.
Signal generation links molecular recognition to measurement. After the antigen-antibody sandwich forms, the enzyme-linked label converts an added substrate into a colored product. Because the color signal is proportional to antigen abundance, comparing signal intensity across samples allows researchers to estimate differences in the target protein. The readout therefore provides more than simple antigen presence or absence.
Antigen capture ELISA is particularly useful when a sample contains many biological components. Immobilizing the capture antibody retains the target at the microplate surface, and detection through a second antibody adds another layer of specificity. These features support sensitive analysis of selected proteins rather than relying on a broad measurement of all proteins present.
An assay workflow begins by immobilizing the capture antibody on a microplate. The biological sample is then applied so its target antigen can bind, followed by the detection antibody to create the sandwich. An enzyme-linked label and substrate generate the measurable color signal. Comparing that signal among samples supports antigen quantification under the same assay design.
The method fits studies that require a specific antigen measurement, including disease diagnosis, pathogen detection, and biomarker studies. It can also support investigations of immune and cellular processes when the target protein reflects those systems. Its quantitative signal enables standardized comparisons across biological samples rather than isolated qualitative observations.
In biology, the assay provides a common measurement framework for evaluating the same target antigen in different samples. Each sample produces a color signal linked to antigen abundance, allowing researchers to compare target levels rather than relying only on visual detection. This standardization is useful for biomarker studies and for examining changes associated with immune or cellular processes.