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Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging
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Developmental Biology
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JoVE Journal Developmental Biology
Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

DOI:
10.3791/64662-v

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09:11 min

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April 28, 2023

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, , ,
1Department of Biological Sciences,University of Arkansas

Chapters

  • 00:05Introduction
  • 00:53Drosophila Embryo Fixation and Devitellinization
  • 03:04Preparing PDMS Wells
  • 03:57Adhering the Embryos to the Coverslips
  • 04:33Activation and Gelation
  • 05:48Digestion and Expansion
  • 06:33Mounting and Imaging
  • 07:08Results: Analysis of Unexpanded and Expanded Embryos
  • 08:45Conclusion

Summary

Automatic Translation

Automatic Translation

Here, a protocol for the implementation of expansion microscopy in early Drosophila embryos to achieve super-resolution imaging using a conventional laser-scanning confocal microscope is presented.

Tags

Keywords: Expansion MicroscopyWhole-mount Drosophila EmbryosSuper-resolution ImagingProtein LocalizationCell MorphologyAgar SlabDouble-sided TapeVitelline MembranePoly-L-LysinePBS Tween

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