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Journal / Developmental Biology / Using Expansion Microscopy to Physically Enlarge…
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Developmental Biology
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JoVE Journal Developmental Biology
Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging
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Using Expansion Microscopy to Physically Enlarge Whole-Mount Drosophila Embryos for Super-Resolution Imaging

Article DOI: 10.3791/64662-v • 09:11 min • April 28th, 2023
April 28th, 2023 •
1, 1, 1, 1
1Department of Biological Sciences, University of Arkansas

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Here, a protocol for the implementation of expansion microscopy in early Drosophila embryos to achieve super-resolution imaging using a conventional laser-scanning confocal microscope is presented.

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Expansion Microscopy Whole-mount Drosophila Embryos Super-resolution Imaging Diffraction Limit Confocal Microscopy Protein Localization Cell Morphology Intact Embryos Subcellular Structures Networks Poly-L-Lysine Agar Slab Petri Dish Double-sided Tape Coated Embryos
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