A subscription to JoVE is required to view this content. Sign in or start your free trial.
1Shanghai Collaborative Innovation Center of Agri-Seeds, School of Agriculture and Biology,Shanghai Jiao Tong University, 2Joint Center for Single Cell Biology, School of Agriculture and Biology,Shanghai Jiao Tong University, 3Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education),Shanghai Jiao Tong University
Chapters
- 00:05Introduction
- 00:36Plant Culture
- 01:20Tissue Preparation and Pretreatment
- 02:37Setting up the Protocol with the Microplate Reader, Establishing the Elicitation System, and Measuring Real-Time ROS Production
- 03:42Results: ROS Induction, the Total Amount of ROS Generated, and ROS Production in the Exposed Cells of the Cut Edge
- 05:07Conclusion
Here, we describe a method for the real-time detection of apoplastic reactive oxygen species (ROS) production in rice tissues in pathogen-associated molecular pattern-triggered immune response. This method is simple, standardized, and generates highly reproducible results under controlled conditions.
