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Journal
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Biology
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从快速冷冻肝组织中分离细胞核用于单细胞多组学
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Isolation of Nuclei from Flash-Frozen Liver Tissue for Single-Cell Multiomics
Please note that all translations are automatically generated.
Click here for the English version.
从快速冷冻肝组织中分离细胞核用于单细胞多组学
DOI:
10.3791/64792-v
•
09:09 min
•
December 09, 2022
•
Mateusz Strzelecki
4
,
Kelvin Yin
,
Carlos Talavera-López
3
,
Celia P. Martinez-Jimenez
4
1
Helmholtz Pioneer Campus (HPC)
,
Helmholtz Munich
,
2
Institute of Computational Biology, Computational Health Department
,
Helmholtz Munich
,
3
Division of Infectious Diseases and Tropical Medicine
,
Ludwig-Maximillian-Universität Klinikum
,
4
TUM School of Medicine
,
Technical University of Munich
Chapters
00:04
Introduction
00:57
Nuclei Isolation
05:11
Nuclei Sorting for Hepatocyte Ploidy Profiling or Well-Based Sequencing Approaches
06:36
Results: Isolation of Nuclei from Flash-Frozen, Archived Liver Tissues for Single-Nucleus RNA-Seq, ATAC-Seq, and Joint Multiomics
08:39
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们提出了一种从快速冷冻,存档的肝组织中分离细胞核的方案,用于单核RNA-seq,ATAC-seq和联合多组学(RNA-seq和ATAC-seq)。
Tags
Nuclei Isolation
Single-cell Multiomics
Density Gradient Centrifugation
Iodixanol
Cell Strainer
Bio-archived Human Liver Samples
Mouse Model
Non-human Primate
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