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Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Visualizing DNA Damage Repair Proteins in Patient-Derived Ovarian Cancer Organoids via Immunofluorescence Assays
DOI:

04:07 min

February 24, 2023

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Chapters

  • 00:04Introduction
  • 00:50Immunofluorescence Staining
  • 02:58Results: Evaluation of DNA Damage Repair Proteins in Organoids
  • 03:34Conclusion

Summary

Automatic Translation

The present protocol describes methods for evaluating DNA damage repair proteins in patient-derived ovarian cancer organoids. Included here are comprehensive plating and staining methods, as well as detailed, objective quantification procedures.

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