Establishment and Culture of Patient-Derived Breast Organoids

Human patient-derived organoids are three-dimensional in vitro model systems that represent both patient diversity and cellular heterogeneity of the tumors. This protocol and video demonstration provide a detailed, hands-on guide to establishing patient-derived breast tumor and normal organoids. Patient-derived breast tumor organoids are exciting new models, but difficult to establish.

The comprehensive protocol we provide here should aid in preparing the researchers attempting to develop breast organoids and acquaint them with the expected challenges. Every PDO line derived from a different patient is unique in morphology and growth rate. Unlike two 2D cell line systems, organoids grow better when plated at a high density, allowing for better intercellular interactions.