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1Innovative Institute of Chinese Medicine and Pharmacy,Chengdu University of Traditional Chinese Medicine, 2Tianfu Chinese Medicine Innovation Harbour,Chengdu University of Traditional Chinese Medicine, 3School of Medical Technology,Chengdu University of Traditional Chinese Medicine, 4China Resources Sanjiu (Ya’an) Pharmaceutical Co., Ltd.
Chapters
- 00:04Introduction
- 01:02Sample Preparation
- 01:55Installation of Desorption Electrospray Ionization (DESI) Unit
- 04:02Desorption Electrospray Ionization‐Mass Spectrometry (DESI‐MS) Image Acquisition
- 05:50Processing DESI‐MSI Data and Visualization
- 06:55Results: Mass Spectrometry Imaging of Root and Whole Leaf Sections
- 07:31Conclusion
In this study, a series of methods are presented to prepare DESI-MSI samples from plants, and a procedure of DESI assembly installation, MSI data acquisition, and processing is described in detail. This protocol can be applied in several conditions for acquiring spatial metabolome information in plants.
