Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

Huizheng Ge; Linlin Zhang; Mengjia Liu; Xi Yang; Xu Wu; Ran Wang; Zhengde Xie

doi:10.3791/64949

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Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection

Standardization of Transfer across Labs between Flow Cytometers for Detection of Lymphocytes in Japanese Encephalitis Vaccinated Children

980 Views

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06:22 min

•

February 10, 2023

DOI:

10.3791/64949-v

DOI:

10.3791/64949-v

•

06:22 min

February 10, 2023

•

947 Views

Huizheng Ge*¹, Linlin Zhang*^2,3, Mengjia Liu*^2,3, Xi Yang¹, Xu Wu¹, Ran Wang^2,3, Zhengde Xie^2,3

¹Center of Excellence (Beijing),Becton Dickinson Medical Devices (Shanghai) Co Ltd ²Beijing Key Laboratory of Pediatric Respiratory Infectious Diseases, Key Laboratory of Major Diseases in Children, Ministry of Education, National Clinical Research Center for Respiratory Diseases, Laboratory of Infection and Virology, Beijing Pediatric Research Institute, Beijing Children’s Hospital,Capital Medical University, National Center for Children’s Health ³Research Unit of Critical Infection in Children, 2019RU016,Chinese Academy of Medical Sciences

Summary

In this study, a method was developed to facilitate the transfer of experimental settings and analysis templates between two flow cytometers in two laboratories for the detection of lymphocytes in Japanese encephalitis-vaccinated children. The standardization method for the flow cytometer experiments will allow research projects to be effectively conducted in multiple centers.