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Automated Multimodal Stimulation and Simultaneous Neuronal Recording from Multiple Small Organisms
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Neuroscience
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JoVE Journal Neuroscience
Automated Multimodal Stimulation and Simultaneous Neuronal Recording from Multiple Small Organisms

Automated Multimodal Stimulation and Simultaneous Neuronal Recording from Multiple Small Organisms

DOI:
10.3791/65042-v

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08:28 min

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March 03, 2023

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, , , ,
1Department of Biomedical Engineering,Worcester Polytechnic Institute, 2Graduate School of Biomedical Sciences,UMass Chan Medical School, 3Department of Biology and Biotechnology,Worcester Polytechnic Institute

Chapters

  • 00:04Introduction
  • 00:48Microfluidic Device Preparation
  • 02:04Animal Loading
  • 03:02Automated Stimulation and Neuronal Recording
  • 04:15Data Analysis Using NeuroTracker
  • 06:08Data Exploration and Visualization
  • 06:47Results: Assessment of Temporal Inhibition, Disinhibition, and Multimodal Stimulation
  • 07:51Conclusion

Summary

Automatic Translation

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We present a method for the flexible chemical and multimodal stimulation and recording of simultaneous neural activity from many Caenorhabditis elegans worms. This method uses microfluidics, open-source hardware and software, and supervised automated data analysis to enable the measurement of neuronal phenomena such as adaptation, temporal inhibition, and stimulus crosstalk.

Tags

Keywords: Automated StimulationMultimodal StimulationSimultaneous Neuronal RecordingMultiple Small OrganismsNeural ResponsesBrain ActivityRepeatabilityNeuronal VariabilityAdaptationSensory IntegrationMicrofluidic DeviceWorm LoadingNeural Phenomena

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