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Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy
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Ultrastructural Localization of Endogenous LC3 by On-Section Correlative Light-Electron Microscopy

Article DOI: 10.3791/65067-v • 11:53 min • March 31st, 2023
March 31st, 2023 •
1, 1, 1, 1, 1, 1, 1
1Center for Molecular Medicine-Cell Biology, University Medical Center Utrecht, Utrecht University

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Summary
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Here, we present a protocol for optimized on-section correlative light-electron microscopy based on endogenous, fluorescent labeling as a tool to investigate the localization of rare proteins in relation to cellular ultrastructure. The power of this approach is demonstrated by ultrastructural localization of endogenous LC3 in starved cells without Bafilomycin treatment.

Tags

Biology Correlative Light-electron Microscopy Autophagic Organelles Electron Microscopy Fluorescence Microscopy Autophagy Proteins Autophagosomes On-section CLEM LC3 Labeling Lysosomal Acidification Autolysosomes
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