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Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

JoVE Journal
Biochemistry

This content is Free Access.

JoVE Journal Biochemistry

Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

Semi-Automated Phenotypic Analysis of Functional 3D Spheroid Cell Cultures

DOI:

10.3791/65086-v

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06:48 min

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August 18, 2023

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Stephanie Stransky, Dejauwne Young, Karoline Mikkelsen³, Annemette Præstegaard Thulesen, Helle Sedighi Frandsen, Simone Sidoli

¹Department of Biochemistry,Albert Einstein College of Medicine, ²Department of Biochemistry and Molecular Biology,University of Southern Denmark, ³CelVivo ApS

Chapters

00:04Introduction
00:36Preparation of Spheroids and Spheroid Culture in Bioreactor
03:00Imaging and Collection of Spheroids
04:28Proteomics Analysis via Liquid Chromatography Coupled with Mass Spectrometry
05:17Results: Proteome Analysis of Liver Spheroids
06:15Conclusion

Summary

Automatic Translation

We present a protocol for growing high-reproducible spheroids and their phenotypic characterization using image capture and proteomics.

Tags

Keywords: 3D Cell Culture Spheroid HepG2/C3A THLE-3 Ultra-low Attachment Plate Bioreactor Proteomic Analysis LC-MS Phenotypic Analysis

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